Tissue culture, which is based on the plant regenerative abilities, has been widely exploited in agricultural technology for more than half a century. Formation of a pluripotent cell mass from detached explant, known as callus, is the first step in tissue culture.Almost all organs of the typical dicot plant Arabidopsis are able to produce callus during their whole life.However,the mature organs of monocot cereal species,such as rice ,maize and wheat.